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The molecules listed below all participate in the reaction catalyzed by the pyruvate dehydrogenase complex (PDC). Select the answer that represents the order of their involvement in the conversion of pyruvate to acetyl-CoA.
I. NAD+
II. Coenzyme A (CoA-SH)
III. Thiamine Pyrophosphate (TPP)
IV. Lipoate (lipoamide)
V. FAD