Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.
a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

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05-12-2022
Biology

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Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.
a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

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jaiswalsaurabh45609 jaiswalsaurabh45609 · Answer 1 · 2022-12-11T23:24:15-05:00

The right order will be: C > B > D > A

Recombinant DNA technology entails changing genetic material outside of an organism to produce living things or their products with improved and desired traits. With the help of the right vector, this approach includes inserting DNA fragments from various sources with desired gene sequences.

In this gene is isolated and inserted into the vector following which recombinants are screened and used for overexpression of protein.

To know more about recombinant DNA technology, click here,

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