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Sagot :
The following steps of the regulation of the trp operon in order of occurrence:
1. RNA polymerase binds to the promotor allowing transcription of the trp genes to proceed.
2. Products of the trp gene synthesize tryptophan.
3. levels of tryptophan rise and more is required.
4. Tryptophan binds to trp repressor protein and induces a conformational change.
5. The trp repressor protein binds to the operator.
6. The trp repressor protein blocks RNA polymerase from binding to the promotor.
What activates the trp operon?
Unless an inducer—lactose—is present in the environment, this operon is always off; lactose causes the production of the genes in this operon. This operon is always expressed unless the corepressor, tryptophan, becomes accessible in the cell. The trp operon is a repressible system.
The amount of free tryptophan in the cell controls the expression of the trp operon through repressor and attenuation mechanisms.
When tryptophan levels are low, the repressor protein separates from the operator sequence, activating the trp operon and enabling RNA polymerase to transcribe the trp genes in the operon.
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