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The beer-lambert law is A = Elc, where A is the absorbance, E is the molar extinction coefficient, l is the path lengh in cm and C is the concentration. The beer lambert law can be expressed in the form of y=mx + b, where A is y, and C is x. What is b and how is it used to calculate the final absorbance? b is the absorbance of another protein and is added from the absorbance of the other A values b is the absorbance of contaminations and is subtracted from the absorbance of the other A values b is the absorbance of the blank and is subtracted from the absorbance of the other A values b is the absorbance of the initial pure protein and is added to the absorbance of the other A values

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