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6 of Part A Which of the following statements about ddNTPs is true? Vlew Available Hint(s) They have an oxygen at the 2' carbon of the sugar. They have a free 3'-hydroxyl group on the sugar. DNA polymerase can add a new DNTP to a 3' ddNTP They have a hydrogen at the 3' carbon of the sugar Submit Part B DNA fragments that are 600 bp long will migrate more quickly through a sequencir gel than fragments that are 150 bp long. Book Pro 6 of 9 Part B DNA fragments that are 600 bp long will migrate more quickly through a sequencing gel than fragments that are 150 bp long. Vlew Available Hint(s) Te False Submit Part C Which of the following statements about manual Sanger sequencing is true? View Available Hint(s) The DNA sequence is read from the top of the gel to the bottom. Each of the four terminating ddNTPs is labeled with a different Book Pro 6 of 9 Part C Which of the following statements about ma Sanger sequencing is true? Vlew Availlable Hint(s) The DNA sequence is read from the top of the gel to the bottom. Each of the four terminating ddNTPs is labeled with a different fluorescent dye. The DNA sequence obtained is complementary to the template strand. One sequencing reaction is performed. Submit Provide Feedback Next > k Pro

Sagot :

1.DdNTPs have a hydrogen at the 3' carbon of the sugar final choice, which is an accurate statement about them.

2. False. Compared to 150 bp-long DNA fragments, 600 bp-long DNA fragments move through a sequencing gel more quickly.

3. The acquired DNA sequence is complementary to the template strand, which is a true statement about manual Sanger sequencing.

4. The acquired DNA sequence is complementary to the template strand, which is a true statement concerning manual Sanger sequencing.

DNA sequencing is the process of determining the nucleic acid sequence, or the arrangement of nucleotides in DNA. Any method or technology can be used to arrange the four bases in this way: adenine, guanine, cytosine, and thymine.Sequencing DNA is the process of determining the locations of the four chemical "bases," or molecular building blocks, that make up the DNA molecule. Researchers can determine what kind of genetic information is stored in a certain DNA segment thanks to the sequence.

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