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Proper Pipetting is an inevitable part of experiments or research conducted under microbiology, biochemistry, and pharmacology-like fields of biology.
Different pipettes are available for various purposes with differing levels of accuracy which are Transfer Pipette, Graduated Pipette, and Single-Channel Pipette.
The equipment is designed to transport a measured volume of liquid with utmost precision. Different pipettes are available for various purposes with differing levels of accuracy. Pipette is a laboratory equipment commonly used in research or lab purposes in various fields of science. Transfer Pipette is considered one of the most basic types of pipette. This is a disposable pipette generally made of plastic generally used for rough measurements. This cannot be used for precise measurements. Graduated pipettes are classified under micropipettes. This is mainly made of long glass tubes. This contains a series of graduations to indicate different calibrated measurements. For measurements using graduated pipettes, we use additional equipment like rubber bulbs at the top of the equipment. This is a non-disposable type instrument that operates by piston-driven air displacement. Single-Channel Pipettes are of the adjustable type that delivers a measured volume of liquid. Also, these pipettes require disposable plastic tips. Many follow mouth piping instead of using rubber bulbs. This must not be appreciated. You should use new rubber bulbs or plastic suction livers for sucking the liquid into the pipette. Overfilling of pipettes using misuse of rubber bulbs may lead to undesirable effects. A lack of attention may lead to the overfilling of the pipette and overflow of the solution. The application of overpressure on pipettes or lack of attention may result in the breakage of glass pipettes and this may cause injuries to your body parts if you're handling it carelessly. Sequential calibration is essential while using an air-displacement pipette.
To learn more about microbiology and biochemisty,
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