if you performed a pcr experiment starting with only one copy of the double-stranded dna , approximately how many dna molecules would be present in the reaction tube after 26 cycles of amplification?

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06-12-2022
Biology

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if you performed a pcr experiment starting with only one copy of the double-stranded dna , approximately how many dna molecules would be present in the reaction tube after 26 cycles of amplification?

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abhinavjh321 abhinavjh321 · Answer 1 · 2022-12-08T13:13:35-05:00

Two strands of DNA are each copied. There are 26 cycles of PCR. Take 2^26 molecules would be present in the reaction tube after 26 cycles of amplification.

After n cycles of PCR's , there are 2n (2 to the n:th power) copies of DNA molecules since each cycle results in the doubling of the number of double-stranded DNA fragments. As an illustration, after 10 cycles, PCR's you have 1024 copies molecules , after 20 cycles, about 1 million copies, etc. There are more than one billion precise duplicates of the original DNA segment after several iterations of the cycle of denaturing and synthesising new DNA. PCR's whole cycle procedure is automated and may be finished in a matter of hours.

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