Outline an experimental approach to determining the average chain growth rate for transcription in vivo. Chain growth rate is the number of nucleotides polymerized per minute per RNA chain. Drag the terms on the left to the appropriate blanks on the right to complete the sentences DNA Label a growing bacterial or cell culture for a brief interval with a mixture of "C-labeled nucleosides which would be converted to nucleotides and incorporated into . Isolate total sucrose-gradient centrifugation and equilibrium density-gradient centrifugation and subject it to hydrolysis with mild Every -terminal nucleotide will be released as a nucleoside. Separate nucleosides from nucleotides, by Total picomoles of nucleotide in the hydrolysate divided by total picomoles of nucleoside gives the number of nucleotides incorporated per interval (minutes) gives the growth rate of individual chains (nucleotides incorporated per growth rate chain-which, when divided by the labeling alkali chain per minute). An interesting variant of the experiment would be to carry it out length with individual radiolabeled nucleosides and ask whether the chain is the same for each ion-exchange chromatography or electrophoresis nucleotide acid RNA microarravs methods reset ?