a researcher is using a vector to introduce a human gene into bacteria. he exposes bacteria to this recombinant molecule and then grows these organisms in culture. he is very surprised to find that all the resulting bacterial colonies are blue and survive treatment with antibiotics. what can he conclude from these results? please choose the correct answer from the following choices, and then select the submit answer button. answer choices all the resulting bacteria contain the human gene but not the plasmid. all the resulting bacteria contain the plasmid without the human gene. all the resulting bacteria contain the recombinant molecule. none of the resulting bacteria contain the recombinant molecule.

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06-12-2022
Biology

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a researcher is using a vector to introduce a human gene into bacteria. he exposes bacteria to this recombinant molecule and then grows these organisms in culture. he is very surprised to find that all the resulting bacterial colonies are blue and survive treatment with antibiotics. what can he conclude from these results? please choose the correct answer from the following choices, and then select the submit answer button. answer choices all the resulting bacteria contain the human gene but not the plasmid. all the resulting bacteria contain the plasmid without the human gene. all the resulting bacteria contain the recombinant molecule. none of the resulting bacteria contain the recombinant molecule.

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contexto1019 contexto1019 · Answer 1 · 2022-12-09T03:29:42-05:00

All of the resulting bacteria contain the pUC18 plasmid without the human gene.

In E. coli, the pUC18 plasmid cloning vector is often employed. Double-stranded circular DNA makes up the molecule (2686 base pairs in length). Large DNA segments may be successfully cloned thanks to pUC18's tiny size. In the right host strains, the pUC18 plasmid induces complement deficiencies in -galactosidase as well as ampicillin resistance.

The MCS region in pUC18 is the opposite of that in pUC19. Both orientations of the restriction enzyme-generated DNA fragments toward the lac promoter can be cloned using pUC18 and pUC19.

Learn more about pUC18 plasmid here:

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