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Using the information you learned in the CRISPR interactive, match the following vocabulary with their respective definitions.

Column A
1.
Targeting:
2.
Binding:
3.
Cleaving:
4.
DNA Repair:

Column B
a. The Cas9 unwinds the DNA double helix to a location that perfectly matches a sequence of 20 nucleotides within the guide RNA. the DNA and matching RNA bind through complementary base pairing.
b. The Cas9-guide RNA complex is introduced into a cell, in which it randomly dissociates with the DNA.
c. Cells contain enzymes that repair double-stranded DNA breaks
d. This step causes the 3D structure to cut DNA strands at a site and upstream.

Sagot :

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