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Low-molecular-mass molecules can be separated using paper chromatography based on how evenly they are distributed in the stationary and mobile phases. Paper chromatography is regarded as a potent analytical technique because of its low cost and the availability of numerous procedures for the separation of chemicals.
An analytical technique for separating cultured compounds or substances is paper chromatography. Since alternative chromatography techniques like thin-layer chromatography have taken its place in the laboratory, it is now largely utilized as a teaching tool (TLC). Two solvents are used in two-dimensional paper chromatography, which rotates the paper 90 degrees between solvents. This is helpful for sorting out complicated mixtures of chemicals with comparable polarity, like amino acids. There are three parts to the setup. A solution that rises the stationary phase as a result of capillary action is known as the mobile phase. The stationary phase typically consists of polar inorganic solvent water, while the mobile phase typically consists of a combination of non-polar organic solvents. In this instance, the stationary phase, water, is supported by paper. Polar water molecules are trapped in the cellulose network of the host paper's empty space. In contrast to paper chromatography, which uses less absorbent paper as its stationary phase, TLC uses a layer of adsorbent (often silica gel or aluminium oxide) as its stationary phase.
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