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What does a large-scale mutation affect? A. the nucleotides with either a substitution, deletion or insertion B. sections in the chromosomes C. one or a few nucleotides in the RNA D. one or a few nucleotides in the DNA

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In general, a large-scale mutation affects "D. one or a few nucleotides in the DNA" which can either be completely random or through certain forces that occur in life. 
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