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Suppose you want to reproduce the indel mutation seen in sequence 2 with higher fidelity after your CRISPR experiment
How would you use CRISPR to generate the same 4 deletion position 33-36 of the shown below?

Reference
Sequence 1 ATGACAGCCATCATCAAAGAGATCGTTAGCAGA GAGATATCAAGAGG
Sequence 2 ATGACAGCCATCATCAAAGAGATCGTTAGCAG- -AAAAGGAGATATCAAGAGG
Sequence 3 ATGACAGCCATCATCAAAGAGATCGTTAGCAG-AACAAAAGGAGATATCAAGAGG
Sequence 4 ATGACAGCCATCATCAAAGAGATCGTTAGCAGAAAGGAGATATCAAGAGG

double stranded DNA at one locus and use NHEJ Cut double stranded DNA at two loci and use NHEJ double stranded DNA at one and use HR double stranded DNA at two loci and use HR

Sagot :

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