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What is the difference in effect that the decolorization agent has on gram positive cell vs gram negative cells?
a. In gram negative cells, the alcohol increases the porosity of the cell wall by dissolving the lipids in the out layers while in gram positive cells the pores are bigger due to more lipids and the ethyl alcohol dehydrates the peptidoglycan layer, causing it to shrink and tighten.
b. In gram positive cells, the alcohol decreases the porosity of the cell wall by retaining the lipids in the out layers while in the gram negative cells, the pores are larger due to more lipids and the ethyl alcohol dehydrates the peptidoglycan layer, causing it to shrink and tighten.
c. In gram positive cells, the alcohol increases the porosity of the cell wall by dissolving the lipids in the out layers while in the gram negative cells, the pores are smaller due to less lipids and the ethyl alcohol dehydrates the peptidoglycan layer, causing it to shrink and tighten.
d. In gram negative cells, the alcohol increases the porosity of the cell wall by dissolving the lipids in the out layers while in gram positive cells the pores are smaller due to less lipids and the ethyl alcohol dehydrates the peptidoglycan layer, causing it to shrink and tighten.