Final answer:
The disk-diffusion method assesses chemical effectiveness against bacteria by measuring zones of inhibition, while use-dilution and in-use tests offer more practical and real-world results in simulating effectiveness under varying conditions.
Explanation:
Disk-Diffusion Method:
- The disk-diffusion method involves applying different chemicals to sterile filter paper disks that are then placed on an agar plate inoculated with bacteria.
- As the chemicals diffuse into the agar, zones of inhibition around the disks indicate the effectiveness of the chemical agent against the bacteria.
- The size of the zones of inhibition correlates to the potency of the chemical agent in inhibiting bacterial growth.
Use-Dilution and In-Use Tests:
- Use-dilution and in-use tests are advantageous compared to the disk-diffusion method as they provide more practical and real-world effectiveness results.
- These tests simulate the actual conditions under which the disinfectants or antiseptics will be used, offering a more accurate representation of their effectiveness.
- Unlike the disk-diffusion method which is more standardized, use-dilution and in-use tests reflect the varying conditions in which these chemical agents are applied.
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