The phage genome integrates into host DNA via site-specific recombination at att sites (present in both the virus and the bacterial host), which is mediated by a virus-encoded integrase.
Lambda DNA is derived from an Escherichia coli bacteriophage and is a linear, double-stranded phage DNA with 12 bp single-stranded complementary 5'-ends (Bacteriophage lambda cI857 Sam7).The DNA molecule has 48502 basepairs and is double-stranded except for the extreme ends. The 5' strand is 12 bases longer than the 3' strand at each end. The end sequences are complementary. It is important to note that DNA transposons do not insert themselves into the genome at random, but rather prefer specific sites. In terms of movement, DNA transposons are classified as autonomous or nonautonomous.
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