coli cell of lacI+lacP+lacOc lacZ-lacY+/lacI-lacP+lacO+lacZ-lacY+will Only express lacY, only in the absence of lactose.
An coli lac operon is a collection of genes ordered in a particular order for lactose metabolism. The lac operon, which comprises of three enzyme-coding genes named lacZ, is activated in the presence of lactose.
the lacI regulatory gene, which codes for repressor proteins, lacY, which codes for lactose permease, lacA, which codes for acetyltransferase, and the lac-galactosidase enzyme. The operator protein is bound by the repressor proteins, which then regulate transcription. It stops transcribing when it binds to the operator.
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