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You are Gram staining a known sample that contains both gram-positive and gram-negative bacteria. After you complete the staining, you observe the following. Which of the following statements would apply to the image you see? A. This image could be explained by omission of the Gram's iodine step, causing all cells to appear gram-negative. B. This image could be explained by leaving the primary stain step on the smear for too long, causing all cells to absorb the red color. C. This image could be explained by omission of the decolorizing step, leading all cells to appear gram-negative. D. This image could be explained by leaving safranin dye on the smear for too long, causing all cells to wash out the purple color You are Gram staining a known sample that contains both gram-positive and gram-negative bacteria. After you complete the staining, you realize that you did not perform the safranin step. Which of the following statements would correctly describe what you would see? A. Gram-negative organisms might incorrectly stain purple. B. Gram-negative organisms might not be visible. C. You would not be able to view any organisms on the slide. D. Gram-positive organisms might incorrectly stain red.

Sagot :

A - This image could be explained by skipping the iodine step in the Gram reaction, which would make all cells appear to be gram-negative.

All cells in a gram stain would be pink if iodine were absent. In bacteria with a thick peptidoglycan wall, the iodine acts as a mordant and maintains the color of the initial crystal violet stain. If iodine were not used, the stain would disappear when alcohol was applied.

B - It's possible that gram-negative creatures are invisible.

After Gram staining, gram negative bacteria show a mild reddish appearance under a light microscope. This is due to the fact that their cell wall's structure prevents them from retaining the crystal violet stain, so they are only colored by the safranin counterstain.

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